Biogas apparatus:

 

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The normal procedure to determine
methanogenic activity involves inoculating a number of vials containing a small
amount of the target media with anaerobic inoculum, incubating them at a
controlled temperature, and periodically checking for the methane production by
manual sampling and determination of the volume of gas released, and analyzing
the gas composition using gas chromatography (GC).

 

Moreover, the biogas produced and its methane
content can only be measured periodically, making it impossible to obtain
satisfactory data to fully understand the degradation dynamics. Therefore,
there is a need for a highly automated methane potential test that allows for
the collection of high quality data, that is less labor-intensive.

 

AMPTS (Automatic Methane Potential Test
System)

 

From the biogas producers’ point of view,
understanding the potential of a substrate to produce methane and its dynamic
degradation profile has a significant impact on the choice of organic
waste/material to digest when producing biogas, as well as provides a better
understanding of the quality of the raw gas produced from a biogas production
facility.

 

 

 

 

 

 

The instrument setup can be divided into
three units: A, B, and C:

 

In the Sample Incubation Unit (unit A),

 

up to 6 or 15 vials can be used containing
small amounts of a sample with anaerobic inoculum are incubated at a desired
temperature. The media in each vial is mixed by a slow rotating agitator.
Biogas is then continuously produced, a parameter which is used to estimate the
bio methanation activity inside each vial.

 

In the CO2-absorbing Unit (unit B),

 

the biogas produced in each vial passes
through an individual vial containing an alkaline solution. Several acid gas
fractions, such CO2 and H2S, are retained by chemical interaction with NaOH,
only allowing CH4 to pass through to the biomethane Gas Volume Measuring
Device. A pH indicator is added into each vial for controlling the acid binding
capacity of the solution.

 

 In
the Gas Volume Measuring Device (unit C),

 

 the
volume of CH4 gas released from unit B is measured using a wet gas flow
measuring device with a multi-flow cell arrangement (6 or 15 cells). This
measuring device works according to the principle of liquid displacement &
buoyancy and can monitor ultra-low gas flows; a digital pulse is generated when
a defined volume of gas flows through the device. An integrated embedded data
acquisition system is used to record, display and analyze the results.

 

 

AMPTS – SAMPLE INCUBATION UNIT

 

 Maximum
number of reactors per system: 15 Reactor materials: glass Standard
reactor volume: 500 ml Power consumption: 1300 W (maximum) Dimension: 50×30×15
cm Temperature control: up to 100 °C (precision 0.2 °C) Mixing in the reactor:
mechanical (adjustable interval and speed), maximum speed 140 rpm AMPTS II

 

 CO2-ABSORPTION
UNIT CO2 absorption bottles: 15 Volume of CO2 absorption bottles:
100 ml Dimension of unit: 44×30×6 cm Absorption liquid: 3 M NaOH with pH
indicator, 80 ml per bottle (not included) Absorption efficiency: > 98%
AMPTS II

 

GAS VOLUME MEASURING DEVICE Working
principle: liquid displacement & buoyancy Up to 15 cells
running in parallel Built-in pressure and temperature sensors Integrated data
acquisition (maximum capacity 2×104 flow cell openings)1 Web based software
running on embedded server Measuring resolution: 10 ml Detection capacity: up
to 13 l cumulative gas per channel for each batch test Measuring range for
instant gas flow rate: 10 to 120 ml per min2 Power consumption: 15 W (average),
28 W (maximum) Dimension: 51×44×18 cm Accuracy: 5% (RE) and Precision: 1% (CV)

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